History of plant cell culture; Media components and preparation; Contamination; Callus induction; Regeneration and morphogenesis; Woody shrubs and trees; Haploid plantas from anther culture; Embryo rescue; Meristem culture for virus-free plants; In vitro propagation for commercial production of ornamentals; Agrobacterium-mediated transformation of plants.
The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area.
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid ...
Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection.