The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection. Comb-bound, three-column, large 9-1/4" x 7-1/2" format Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts Necessary reagents and equipment are presented in a checklist at the beginning of each protocol Techniques for bacteria are complemented with those for Drosophila Each experiment has been tested in the laboratory by students for five years Features a complete chapter on computers in the molecular biology laboratory Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains
L. Covarrubias and F. Bolivar, Gene 17, 79 (1982). M. Kahn, R. Kolter, C. Thomas, D. Figurski, K. Meyer, E. Remaut, and D. Helinski, Methods Enzymol. 68,268 (1979). J. E. L. Larsen, K. Gerdes, J. Light, and L. Molin, Gene 28, 45 (1984).
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression.
BioBuilder BioBuilder is a short practical course in synthetic biology for high school or early university levels, is based upon prize-winning iGEM projects and is available online: http://www.biobuilder.org/activities/ Detailed ...
In yeast , for example , gene expression patterns obtained using cDNA arrays correlate very well with changes in the yeast cell cycle ( yeast data sets and analysis tools are available at cmgm . stanford . edu / pbrown / ) .
This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering.
This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription ...
This fourth edition of "The Mouse Manual¨is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology.
This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to ...