Recombinant DNA Technology is focuses on the current state of knowledge on recombinant DNA technology and its applications. The genome is the genetic material of an organism, that is, the total amount of DNA in the cell. In eukaryotes, it is usually organized into a set of chromosomes, which are extremely long chains of DNA that are highly condensed. In the picture below, human DNA is shown packaged into chromosome units (as seen during mitotic metaphase). Note the sister chromatids (that contain identical daughter DNA molecules), centromeres and telomeres. Recombinant DNA technology, joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Since the focus of all genetics is the gene, the fundamental goal of laboratory geneticists is to isolate, characterize, and manipulate genes. Although it is relatively easy to isolate a sample of DNA from a collection of cells, finding a specific gene within this DNA sample can be compared to finding a needle in a haystack. A gene is a segment of nucleic acid that contains the information necessary to produce a functional product, usually a protein. The genetic analysis of entire genomes is called genomics. Such a broadscale analysis has been made possible by the development of recombinant DNA technology. In humans, knowledge of the entire genome sequence has facilitated searching for genes that produce hereditary diseases. Genes consist of a long strand of DNA (RNA in some viruses) that contains a promoter, which controls the activity of a gene, and a coding sequence, which determines what the gene produces. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology.