Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual
- ISBN-10
- 0124095933
- ISBN-13
- 9780124095939
- Category
- Science
- Pages
- 266
- Language
- English
- Published
- 2014-01-11
- Publisher
- Academic Press
- Authors
- Jack Kyte, Thomas E. Crowley
Description
Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects Provides instructional guidance for students to design their own experiments Includes advanced analytical techniques Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH Includes access to a website with additional resources for instructors
Get the book
Similar books
-
Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual
By Jack Kyte, Thomas E. CrowleyInstructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater ...
-
Methods to Determine Enzymatic Activity
By Alane Beatriz Vermelho, Sonia CouriThe book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text.
-
Chemistry and Methods of Enzymes
By James B. Sumner, G. Fred SomersEhrlich, 121, 130 Ehrlich, F., 231, 337 Eichel, B., 231, 232, 235 (130), 237, 239, 260 (44), 273 Eiler, J. J., 98, ... 41), 193 Engel, W., 292 (88), 298 Engelhardt, W. A., 95 (60, 58), 96 (60), 97 (60), 100 Engelhardt, W. A., 360, ...
-
Biocatalysis for Practitioners: Techniques, Reactions and Applications
By Gonzalo de Gonzalo, Iván LavanderaThis reference book originates from the interdisciplinary research cooperation between academia and industry.
-
Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction Intermediates
By Daniel L. PurichNow with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.
-
Application of Proteolytic Enzymes to Protein Structure Studies
By Elemer MihalyiApplication of Proteolytic Enzymes to Protein Structure Studies
-
Asymmetric and Selective Biocatalysis
By Jose M. Palomo, Cesar MateoThe dependence of OYEs on pyridine nucleotide coenzyme can be avoided by using nicotinamide coenzyme mimetics. In the review, three main classes of OYEs are described and characterized.
-
Undergraduate Research in the Sciences: Engaging Students in Real Science
By Sandra Laursen, Anne-Barrie Hunter, Elaine SeymourHowever, the lack of systematic data collection by institutions is a problem: as Kenny (2003) points out, “A lot may be happening, but no one is charged with keeping score” (p. 105). Several indicators reflect growing interest in UR by ...
-
For the Love of Enzymes: The Odyssey of a Biochemist
By Arthur KornbergFor the Love of Enzymes: The Odyssey of a Biochemist
-
Fitting Models to Biological Data Using Linear and Nonlinear Regression: A Practical Guide to Curve Fitting
By Harvey Motulsky, Arthur ChristopoulosThis book, by the author of the very successful Intuitive Biostatistics, addresses this relatively focused need of an extraordinarily broad range of scientists.