Chemistry and Methods of Enzymes, Third Edition focuses on the processes, methodologies, and reactions in enzyme chemistry, as well as kinetics, nucleases, esterases, and carbohydrates. The publication first underscores the general properties of enzymes, including chemical nature, occurrence, numerical characterization of enzyme concentration, kinetics of enzyme reactions, preparation of commercial enzymes, purification and preservation of enzymes, relations of vitamins to enzymes, and zymogens and kinases. The text then takes a look at esterases and carbohydrates. Topics include pectin depolymerase, heparinase, xylanase, chitinase, dextranase, trehalase, nucleotide phosphatases, glucosulfatase, and gastric lipase. The manuscript examines nucleases, nuclein deaminases, amidases, proteolytic enzymes, and hydrases. Discussions focus on enolase, aconitase, peptidases as metalloproteins, glutaminases, aspartase, urease, adenosine deaminase, and nucleoside phosphorylase. The book also elaborates on iron and copper enzymes, dehydrogenases containing coenzymes I and II, and yellow enzymes. The text is a dependable source of data for chemists and researchers wanting to dig deeper into the chemistry and methods of enzymes.
This book covers the most recent development of enzymatic organic synthesis, with particular focus on the use of isolated enzymes.
Grind 50 mg. of vacuum-dried firefly lanterns“ (10 or 12 lanterns) with 5 ml. ice-cold 0.] M Na arsenate buffer“) or Na phosphate buffer“ (pH 7.4) for 5 to 10 min. Filter the mixture into a test tube standing in an ice bath.
Methods of Enzymatic Analysis
Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry This book aims to make biocatalysis more ...
In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes.
Exploring the theories, methodologies and applications in simulations of enzymatic reactions, this book is a great resource for postgraduate students and researchers.
Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater ...
Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional ...
This important book: Offers a guide to supported and therefore recyclable catalysts, which is one of the most important tools for developing a highly sustainable chemistry Presents various immobilization techniques and applications Explores ...
Biotechnol. Bioeng., 16, 99. Kohen, A. (2006). In A. Kohen, & H.-H. Limbach (Eds.), Isotope Effects in Chemistry and Biology (p. 744). Boca Raton, Florida: Taylor and Francis. Kohen, A., & Klinman, J. P. (1999). Chem. Biol., 6, R191.