Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media.
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid ...
This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and ...
Plant Tissue Culture: Techniques and Experiments
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry.
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields.
Plant Tissue Culture and Its Bio-technological Application: Proceedings of the First International Congress on Medicinal Plant Research, Section B, held...
All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published.
Haberlandt ́s vision of the totipotency of plant cells represents the actual beginning of tissue culture. This book pays homage to a great Austrian scientist and the further development of his ideas.
However, this edition still maintains the integration that was characteristic of the previous editions. The first volume of the new edition highlights the scientific background of in vitro propagation.