Laboratory Methods in Enzymology: Protein Part A

Laboratory Methods in Enzymology: Protein Part A
ISBN-10
0124200702
ISBN-13
9780124200708
Series
Laboratory Methods in Enzymology
Category
Science
Language
English
Published
2014-01-31
Publisher
Academic Press

Description

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradation Labeling a protein with fluorophores using NHS ester derivitization Immunoaffinity purification of proteins Proteolytic affinity tag cleavage Purification of GST-tagged proteins

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