Protein Engineering: Methods and Protocols
- ISBN-10
- 1493973649
- ISBN-13
- 9781493973644
- Series
- Protein Engineering
- Category
- Science
- Pages
- 350
- Language
- English
- Published
- 2017-10-30
- Publisher
- Humana Press
- Authors
- Uwe T. Bornscheuer, Matthias Höhne
Description
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
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Other editions
-
- 2013-01-01
- 221 pages
- Paperback
- Nova Science Pub Incorporated
-
- 2012-12-02
- 440 pages
- Ebook
- Elsevier
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