Proteomics in Practice: A Guide to Successful Experimental Design
- ISBN-10
- 3527622306
- ISBN-13
- 9783527622306
- Category
- Science
- Pages
- 502
- Language
- English
- Published
- 2008-09-08
- Publisher
- John Wiley & Sons
- Authors
- Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Description
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
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